Chicken tachyzoites antigen based ELISA can successfully diagnose toxoplasmosis


Chicken tachyzoites antigen based ELISA can successfully diagnose toxoplasmosis

Toxoplasma gondii is a successful food and water borne parasite that infects approximately 1 billion people worldwide1. This parasite is responsible for a disease which is known as “toxoplasmosis”.

Accordingly, chicken meat is regarded as the most significant part of kitchen, as it is consumed extensively around the world. Consequently, use of uncooked or not properly cooked poultry meat can pose a risk factor for T. gondii infection of human as well as animals2, which is an alarming situation.

Chicken is known to be the vital intermediate host of Toxoplasma gondii and it can shed millions of infective resistance oocyst on the ground surrounding human household. Free rang chicken feeds on the ground usually, therefore, the occurrence of T. gondii in chicken is a suitable indicator of soil contamination with T. gondii oocysts3.

Considering these facts, researchers got motivated and decided to design a new experiment in order to isolate theT. gondii tachyzoites from free rang chicken. Moreover, during this study scientists also evaluated its potency in diagnosis of human as well as chicken toxoplasmosis4.

During this study, researchers prepared the crude extract of Toxoplasma gondii tachyzoites from chicken local isolate. Afterwards, they were employed to examine chicken toxoplasmosis by means of enzyme-linked immunosorbent assay “ELISA”.

This extractwas found to be successful in detection of Toxoplasma antibodies in 275 out of 352 free rang chicken serum samples (under examination). Furthermore, this vital side of the present study is adoption of crude chicken tachyzoites antigen in diagnosis of human toxoplasmosis, where it proved 100% sensitivity in the detection of Toxoplasma antibodies in all examined cases (61 samples) of human toxoplasmosis.

Conclusively, chicken tachyzoites antigen based ELISA can be effectivelyused to diagnose chicken as well as human toxoplasmosis. Moreover, this research will sharpen the horizons towards new phenomenon regarding toxoplasmosis and its causal agent “Toxoplasma gondii”.In addition, results of this study are helpful to control and treat this imperative disease.


  1. Xiao, J. and R.H. Yolken, 2015. Strain hypothesis of Toxoplasma gondii infection on the outcome of human diseases. ActaPhysiologica, 213: 828-845.
  2. Dubey, J.P., 2010. Toxoplasma gondii infections in chickens (Gallus domesticus): Prevalence, clinical disease, diagnosis and public health significance. Zoonoses Public Health, 57: 60-73.
  3. Dubey, J.P. and J.L. Jones, 2008. Toxoplasma gondii infection in humans and animals in the United States. J. Parasitol., 38: 1257-1278.
  4. Soad E. Hassan, Nagwa I. Toaleb, Eman E. El Shanawany, Raafat M. Shaapan, Sanaa K.A. Abou-El-Dobal and Ehab Kotb Elmahllawy, 2016. Diagnosis of Human Toxoplasmosis Using Toxoplasma gondii Tachyzoites Crude Antigen of Different Origin. J. Zool. Res., 12: 11-17.