Flow cytometry for the identification of genome size in R. macrophylla

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Flow cytometry for the identification of genome size in R. macrophylla

Classification of plants has helped scientific research. In classification, plants are given scientific names that are universal. Scientific names remain unchanged in any language. The scientific study of plants leading to classification is a continuous process. Everyday scientific research is ongoing to further identify properties of different plants.

Plants are assigned different genera based on fundamental similarities. Variation existing between different genera of the same species makes each plant unique.

Scientific research of each plant starts from its DNA study. Extract from the plant under study is obtained. It is subjected to processing for DNA study. Different techniques are adopted to visualize the genome of a plant. Such thorough scientific research helps in better understanding of the plant species and genus. Observation of fundamental similarities during DNA studies leads to allotment under the same genus of different species.

Rosa macrophylla is an attractive subject for DNA study in plants due to its small genome size. Science has yet to establish the Rose classical genetic. Muhammad Idrees and his team of researchers from China studied the genome size of R. macrophylla.

For this study, they established flow cytometry as the most suitable technique for study of plant genome size. Flow cytometry is a fast and throughput technique. It provides a convenient and rapid nuclei analysis. It was developed by Galbraith. The technique involves cutting plant tissue with a razor blade in a buffer solution. Since its development, many researchers have manipulated the process to reveal different outcomes. For the study of plants’ nuclear DNA, the material is stained with propidium iodide. The team of researchers developed a suitable protocol for the assessment of DNA contents in R. macrophylla using flow cytometry.

The team conducted experiments at Chengdu Institute of Biology, Chinese Academy of Science, Chengdu (Sichuan), China. The duration was 10 months from October, 2016 to November, 2017.

The methodology of Galbraith was followed for the research study. 20-50mg of freshly chopped leaf tissue was used to prepare samples. They were analyzed within 15 minutes. The research concluded that the methods used were able to accurately investigate DNA. The team published their research article in the Asian Journal of Plant Sciences.